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KMID : 0385520170300030130
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Analytical Science & Technology
2017 Volume.30 No. 3 p.130 ~ p.137
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Development of HPLC method for differentiation of three parts of mulberry tree
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Eom Ji-Hyun
Vu Thi Phuong Duyen
Cai Linxi
Zhao Yan
Li Hong Xu
Yang Seo-Young
Kim Young-Ho
Kim Seok-Jin
Cho Hyun-So
Bao Haiying
Chem Jianbo
Kim Kyung-Tae
Kang Jong-Seong
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Abstract
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The leaves (Mori Folium; MF), branches (Mori Ramulus; MR), and root bark (Mori Cortex Radicis; MCR) of the mulberry tree have been used as therapeutic herbs for centuries. Existing analytical methods were developed specifically for different parts of the tree and cannot be applied to samples containing a mixture of tree parts. Such method specialization is time-consuming and requires separate identification and quality control of each tree part. This report describes an HPLC method for the simultaneous quality control and discrimination of MF, MR, and MCR using four marker compounds: rutin, kuwanon G, oxyresveratrol, and morusin. An Optimapak column (, ) was used with a gradient elution of 0.1 % formic acid in water and acetonitrile. The flow rate was 1.0 mL/min and the detection wavelength was 270 nm. In quantitative analyses of the three parts, rutin (0.11 % w/w) was detected only in MF. The oxyresveratrol content (0.12 % w/w) was highest in MR. Kuwanon G (0.33 % w/w) and morusin (0.18 % w/w) were higher in MCR than in other parts. The HPLC method given herein can be used to simultaneously classify and quantify three herbal medicines from the mulberry tree.
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KEYWORD
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HPLC, mori folium, mori ramulus, mori cortex radicis, simultaneous analytical method
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